Translational_Unit

Part:BBa_K3997008:Design

Designed by: Nixue Song   Group: iGEM21_WFLA_YK_PAO   (2021-10-12)


pET28a-IsPETase-MHETase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 510
    Illegal PstI site found at 1671
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 153
    Illegal NheI site found at 466
    Illegal PstI site found at 1671
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1554
    Illegal XhoI site found at 1033
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 510
    Illegal PstI site found at 1671
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 510
    Illegal PstI site found at 1671
    Illegal NgoMIV site found at 1239
    Illegal NgoMIV site found at 1353
    Illegal NgoMIV site found at 1809
    Illegal AgeI site found at 789
    Illegal AgeI site found at 1423
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No


Source

No

References